EFFECT OF PHYTOHORMONES AND THEIR DIVERSE CONCENTRATIONS ON REGENERATION OF ROSE (ROSA HYBRIDA L.)

Abstract

Roses most important regularly used for ornamental, medicinal and aromatic rationale in the world. The relevance of plant tissue culture technology to produce planting material of rose in masses depends on the availability of an effective regeneration protocol. The present experiment was done to scrutinize for appropriate basal medium of Murashige and Skoog (1962), phytohormones with their diverse concentrations influence for establish In vitro shoot and root induction of rose (Rosa hybrida L.). The statically analysis of variation explain that least days to initiation, number of shoots, length of shoot cm, number of leaves, days taken in root initiation and number of roots were significant @ 5% possibility. Increase evidence viewing that experimental conclusion exhibit that minimum days to initiation, utmost number of shoots bottle-1 , shoot length bottle-1 and number of leaves bottle-1 be record within the concentration of MS + NAA 0.5 mgL-1 + BAP 2 mgL-1 . Hence forward minimum days taken in root initiation, highest roots number recorded at 1/2MS + NAA 1.0mg/l + IBA 1.0 mg/l respectively. In vitro healthy and complete plantlets successfully were shifted in to different pot mixtures, supreme survival % recorded at Soil+sand+FYM (1:1:1).

Keywords

• regeneration
• (rosa hybrida l.)
• phytohormones
• concentrations
• acclimatization