Generation of three iPSC lines from fibroblasts of a patient with Aicardi Goutières Syndrome mutated in TREX1

Rosalba Monica Ferraro; Gaetana Lanzi; Stefania Masneri; Chiara Barisani; Giovanna Piovani; Giulia Savio; Marco Cattalini; Jessica Galli; Cristina Cereda; Marco Muzi-Falconi; Simona Orcesi; Elisa Fazzi; Silvia Giliani

Stem Cell Research (Dec 2019)

Generation of three iPSC lines from fibroblasts of a patient with Aicardi Goutières Syndrome mutated in TREX1

Rosalba Monica Ferraro,
Gaetana Lanzi,
Stefania Masneri,
Chiara Barisani,
Giovanna Piovani,
Giulia Savio,
Marco Cattalini,
Jessica Galli,
Cristina Cereda,
Marco Muzi-Falconi,
Simona Orcesi,
Elisa Fazzi,
Silvia Giliani

Affiliations

Rosalba Monica Ferraro: ''Angelo Nocivelli'' Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, Italy, ASST Spedali Civili, Brescia, Italy; Corresponding author.
Gaetana Lanzi: ''Angelo Nocivelli'' Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, Italy, ASST Spedali Civili, Brescia, Italy
Stefania Masneri: ''Angelo Nocivelli'' Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, Italy, ASST Spedali Civili, Brescia, Italy
Chiara Barisani: ''Angelo Nocivelli'' Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, Italy, ASST Spedali Civili, Brescia, Italy
Giovanna Piovani: Biology and Genetics Division, Department of Molecular and Translational Medicine, University of Brescia, Italy
Giulia Savio: Biology and Genetics Division, Department of Molecular and Translational Medicine, University of Brescia, Italy
Marco Cattalini: Pediatric Clinic, Department of Clinical and Experimental Sciences, University of Brescia, Italy, ASST Spedali Civili, Brescia, Italy
Jessica Galli: Unit of Child Neurology and Psychiatry, ASST Spedali Civili, Brescia, Department of Clinical and Experimental Sciences, University of Brescia, Italy
Cristina Cereda: Center of Genomic and Post-Genomic, IRCCS Mondino Foundation, Pavia, Italy
Marco Muzi-Falconi: Department of Biosciences, University of Milano, Italy
Simona Orcesi: Department of Brain and Behavioral Sciences, University of Pavia, Italy, Child Neurology and Psychiatry Unit, IRCCS Mondino Foundation, Pavia, Italy
Elisa Fazzi: Unit of Child Neurology and Psychiatry, ASST Spedali Civili, Brescia, Department of Clinical and Experimental Sciences, University of Brescia, Italy
Silvia Giliani: ''Angelo Nocivelli'' Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, Italy, ASST Spedali Civili, Brescia, Italy

Journal volume & issue: Vol. 41

Abstract

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Fibroblasts from a patient with Aicardi Goutières Syndrome (AGS) carrying a compound heterozygous mutation in TREX1, were reprogrammed into induced pluripotent stem cells (iPSCs) to establish isogenic clonal stem cell lines: UNIBSi006-A, UNIBSi006-B, and UNIBSi006-C. Cells were transduced using the episomal Sendai viral vectors, containing human OCT4, SOX2, c-MYC and KLF4 transcription factors. The transgene-free iPSC lines showed normal karyotype, expressed pluripotent markers and displayed in vitro differentiation potential toward cells of the three embryonic germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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