LncRNA RBPMS-AS1 promotes NRGN transcription to enhance the radiosensitivity of glioblastoma through the microRNA-301a-3p/CAMTA1 axis

Wenyang Li; Yan Cui; Wenjia Ma; Ming Wang; Yang Cai; Yugang Jiang

Translational Oncology (Jan 2022)

LncRNA RBPMS-AS1 promotes NRGN transcription to enhance the radiosensitivity of glioblastoma through the microRNA-301a-3p/CAMTA1 axis

Wenyang Li,
Yan Cui,
Wenjia Ma,
Ming Wang,
Yang Cai,
Yugang Jiang

Affiliations

Wenyang Li: Department of Neurosurgery, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China
Yan Cui: Department of Neurosurgery, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China
Wenjia Ma: Department of Neurosurgery, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China
Ming Wang: Department of Neurosurgery, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China
Yang Cai: Department of Neurosurgery, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China
Yugang Jiang: Department of Neurosurgery, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China; Corresponding author at: Department of Neurosurgery, Second Xiangya Hospital of Central South University, No. 139, Mid Renmin Road, Furong District, Changsha, Hunan, 410011, PR China.

Journal volume & issue: Vol. 15, no. 1
p. 101282

Abstract

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Objective: Glioblastoma (GBM) is the most frequent brain malignancy with high incidence, and long noncoding RNAs (lncRNAs) exerts functions in GBM. In this research, we focused on the capabilities of lncRNA RBPMS-AS1 in radiosensitivity of GBM. Methods: RBPMS-AS1 and CAMTA1 expression levels were determined in GBM tissues and cells. StarBase v3.0 database was searched for predicting miRNAs that simultaneously bound to RBPMS-AS1 and CAMTA1. pcDNA3.1-RBPMS-AS1, pcDNA3.1-CAMTA1, miR-301a-3p mimic, or pcDNA3.1-RBPMS-AS1/pcDNA3.1-CAMTA1 and miR-301a-3p mimic were transfected into GBM cells to test radiosensitivity, cell proliferation and apoptosis. The interactions of miR-301a-3p with RBPMS-AS1 and CAMTA1, as well as CAMTA1 and NRGN, were confirmed. In vivo imaging technology was utilized to detect tumor growth in orthotopic xenograft tumors, and Ki67 expression was tested in intracranial tumors. Results: RBPMS-AS1 and CAMTA1 levels were reduced in GBM tissues and cells. miR-301a-3p had a binding site with both RBPMS-AS1 and CAMTA1 and it was the most significantly-upregulated one. Upregulation of RBPMS-AS1 or CAMTA1 enhanced the radiosensitivity and cell apoptosis while suppressing proliferation of GBM cells. Conversely, miR-301a-3p overexpression diminished the radiosensitivity and cell apoptosis while inducing proliferation of GBM cells. Overexpression of RBPMS-AS1 or CAMTA1 reversed the effects of overexpressed miR-301a-3p in GBM cells. Mechanistically, RBPMS-AS1 enhanced CAMTA1 expression in GBM cells through sponging miR-301a-3p, and CAMTA1 promoted NRGN expression. In animal experiments, overexpressed RBPMS-AS1 inhibited tumor growth and the positive expression of Ki67 both before and after radiation therapy. Conclusion: RBPMS-AS1 promotes NRGN transcription through the miR-301a-3p/CAMTA1 axis and enhances the radiosensitivity of GBM.

Published in Translational Oncology

ISSN: 1944-7124 (Print); 1936-5233 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.journals.elsevier.com/translational-oncology/

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