Heliyon (Jun 2022)
Techniques for bone assessment and characterization: porcine hard palate case study
Abstract
The hard palate plate has an important structural function that separates the nasal cavity and the nasopharynx. Incomplete regeneration of palatal fistulae in children with a cleft palate deformity after primary palatoplasty is a relatively common complication. To date, the information about the physicochemical bone features of this region is deficient, due to the low availability of human samples. Swine and human bone share anatomical similarities. Specifically, pig bones are widely used as experimental animal models in dental, orthopedic, or surgical techniques. The aim of this study was to show different techniques to evaluate and characterize alternative properties of pig hard palate bone, compared to commercial hydroxyapatite, one of the most used biomaterials for bone tissue regeneration. Chemical analyses by Energy dispersive spectroscopy (EDS) and X-ray fluorescence (XRF) showed calcium and phosphate ions as the main constituents of bone, while magnesium, iron, sodium, potassium, and zinc ions were minor constituents. The calcium phosphate molar ratio (Ca/P) in the bone was low (1.1 ± 0.2) due to the very young specimen sample used. The FTIR spectrum shows the presence of phosphates ions (PO43-) and the main characteristics of collagen type I. The XRD results showed that the hard palate bone has a mixture of calcium, octacalcium dihydrogen phosphate (OCP), and apatite, where OCP is the predominant phase. Besides, this research demonstrated that the young bone has low crystallinity and small crystal size compared with commercial hydroxyapatite (HA). The palatine process of maxilla density and porosity data reported, suggest that the palate bone is getting closer to the compact bone with a 52.78 ± 2.91% porosity and their mechanical properties depend on the preparation conditions and the area of the bone analyzed.
