Doped-MXene assists in deciphering metabolic signature of psoriasis and unraveling dysregulated leukotriene metabolism

Abstract

Psoriasis, characterized by erythematous plaques, desquamation, persistent pruritus, and discomfort, significantly compromises the quality of life for afflicted individuals. The intricate interplay of immunological, genetic, and environmental factors has left the molecular intricacies of psoriasis only partially elucidated, underscoring the imperative to unravel these intricate mechanisms. In contradistinction to conventional techniques such as nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS), the burgeoning laser desorption/ionization (LDI) metabolic paradigm holds substantial promise in elucidating the molecular mechanism of psoriasis. Nonetheless, achieving heightened sensitivity and high-throughput metabolite detection within intricate clinical biosamples mandates stringent requisites, encompassing favourable ionization efficiency and high surface area. Henceforth, we proffered a dual-strategy entailing the utilization of transition metal carbides (MXene) with unique morphology and the integration of multi-walled carbon nanotubes (MWCNTs) to ameliorate these challenges for psoriasis. We have corroborated the morphological architecture, elemental composition, and efficacy of LDI when applied to MWCNTs-doped MXene. Egregiously, through the employment of both animal models and clinical specimens, we attained diagnostic efficiencies of 0.959 and 0.924 via receiver operating characteristic (ROC) curve analysis, respectively, for discrimination psoriasis from control individuals. Doped MXene evinced exceptional signal amplification and imperviousness to salt and protein interference. Furthermore, enrichment pathway analysis unveiled a significant escalation in leukotriene E4 and arachidonic acid levels within the psoriasis cohort. Harnessing LDI metabolic fingerprints, concomitant with MWCNTs-doped MXene, expedited the recognition of the distinct metabolic characteristics inherent to psoriasis, thereby laying bare the dysregulation in leukotriene metabolism. This epochal breakthrough holds immense potential to usher in new vistas for exploring the underlying mechanisms governing the onset and progression of psoriasis-related disorders, thereby significantly contributing to the refinement of psoriasis health management strategies.

Keywords

• Doped MXene
• Metabolic signature
• Psoriasis
• Dysregulated leukotriene metabolism