STUDY OF TAXONOMICAL AND IDENTIFICATION FROM SOME PHENOLIC FOR SOME PLANT SPECIES GROWING AT ATRUSH REGION NORTHERN IRAQ USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Abstract

This study was carried out in the laboratory of collage of agriculture and Forestry to identifying the phenolic compounds for ten kinds of plants of the species used in plant identification, The crude lipid free extract was prepared by using soxhlet and Petroleum ether and Ethanol as a solvent, HPLC High performers liquid Chromatography was used for determining phenolic compounds in the extract. The results showed the isolation of 8phenolic compounds including (Hydroquino, Salicylis acid, Coumarin, Cinnamic acid, Thymol,Quercetin, Rutin,Gallic acid), The results were showed many differences in the number and rates of these phenolic compounds between the species, Salicylic acid was the purulent in species of Fabaceae family,(Trifolium purpureum, T. campestre,T. palaestin),foure importent phenolic compounds in the Asteraceae family differ according to the species (Centaurea pallascens, Centaurea solstitialis, Centaurea calcitrapa)in clouding (Quercetin, Coumarin, Cinnamic acid, Salicylic acid), while in Fagaceae family Quercus infectoria four types of phenolic compounds were identified, Rutin was the only identified of the phenolic compounds, the remaning compounds were unknown glycosides,while in Quercus aegilops two chemical compounds(Quercietin and Salicylic acid) were identified. These differences in chemical compounds could be used as an indicator for plant identification .

Keywords

• : plant identification
• plants species
• sequenitial extraction
• phenolic compounds