Spectroscopic investigation and chemical fingerprint of Datura innoxia dry biomass

Abstract

In this study it is presented the application of spectroscopic methods, such as fluorescence study and attenuated total reflection Fourier transform infrared analysis for identification and detection of secondary metabolite specially, hyoscine, and organic functional groups from dry biomass of Datura innoxia vegetative organs. The experimental results confirmed the presence of some important classes of secondary metabolites useful in medicine domain, e.g., alkaloids, terpenes, phenolics, sterols, carbohydrates. Regarding fluorescence study, maximum excitation wavelengths for hyoscine were found to be 345 nm (1-butanol and ethanol). Moreover, the results showed the presence of hyoscine in all structural units which is in according with other studies.

Keywords

• FTIR-ATR
• Fluorescence spectroscopy
• Secondary metabolites
• Tropane alkaloids
• Hyoscine
• Soxhlet method