Nature Communications (Mar 2021)
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
- Yingxiao Zhang,
- Qiurong Ren,
- Xu Tang,
- Shishi Liu,
- Aimee A. Malzahn,
- Jianping Zhou,
- Jiaheng Wang,
- Desuo Yin,
- Changtian Pan,
- Mingzhu Yuan,
- Lan Huang,
- Han Yang,
- Yuxin Zhao,
- Qing Fang,
- Xuelian Zheng,
- Li Tian,
- Yanhao Cheng,
- Ysa Le,
- Bailey McCoy,
- Lidiya Franklin,
- Jeremy D. Selengut,
- Stephen M. Mount,
- Qiudeng Que,
- Yong Zhang,
- Yiping Qi
Affiliations
- Yingxiao Zhang
- Department of Plant Science and Landscape Architecture, University of Maryland
- Qiurong Ren
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Xu Tang
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Shishi Liu
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Aimee A. Malzahn
- Department of Plant Science and Landscape Architecture, University of Maryland
- Jianping Zhou
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Jiaheng Wang
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Desuo Yin
- Department of Plant Science and Landscape Architecture, University of Maryland
- Changtian Pan
- Department of Plant Science and Landscape Architecture, University of Maryland
- Mingzhu Yuan
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Lan Huang
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Han Yang
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Yuxin Zhao
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Qing Fang
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Xuelian Zheng
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Li Tian
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Yanhao Cheng
- Department of Plant Science and Landscape Architecture, University of Maryland
- Ysa Le
- Department of Plant Science and Landscape Architecture, University of Maryland
- Bailey McCoy
- Department of Plant Science and Landscape Architecture, University of Maryland
- Lidiya Franklin
- Department of Plant Science and Landscape Architecture, University of Maryland
- Jeremy D. Selengut
- Center for Bioinformatics and Computational Biology, University of Maryland
- Stephen M. Mount
- Department of Cell Biology and Molecular Genetics, University of Maryland
- Qiudeng Que
- Syngenta
- Yong Zhang
- Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
- Yiping Qi
- Department of Plant Science and Landscape Architecture, University of Maryland
- DOI
- https://doi.org/10.1038/s41467-021-22330-w
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 11
Abstract
CRISPR-Cas12a is a promising system for targeting AT-rich regions of the genome. Here the authors identify Cas12a orthologs with expanded targeting scope and develop a highly multiplexable editing system in rice.
