Correlation between gut microbiota characteristics and non-small cell lung cancer based on macrogenomics sequencing
GuiLin Zeng,
LiRong Zeng,
Ying Wang,
Zhi Cao,
XiangHua Zeng,
ZhiHong Xue,
ShiLan Liu,
YaMao Li,
Lang He
Affiliations
GuiLin Zeng
Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People’s Hospital, University of Traditional Chinese Medicine)
LiRong Zeng
Chengdu Railway Health School
Ying Wang
Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People’s Hospital, University of Traditional Chinese Medicine)
Zhi Cao
Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People’s Hospital, University of Traditional Chinese Medicine)
XiangHua Zeng
Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People’s Hospital, University of Traditional Chinese Medicine)
ZhiHong Xue
Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People’s Hospital, University of Traditional Chinese Medicine)
ShiLan Liu
Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People’s Hospital, University of Traditional Chinese Medicine)
YaMao Li
Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People’s Hospital, University of Traditional Chinese Medicine)
Lang He
Department of Oncology, The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu, Cancer Prevention and Treatment Institute of Chengdu, Chengdu Fifth People’s Hospital, University of Traditional Chinese Medicine)
Abstract Objective Non-small cell lung cancer (NSCLC) patients undergoing chemotherapy and immunotherapy experience disturbances in the gut microbiota. This study intends to find out the correlation between gut microbiota and clinical indices before and after radiotherapy for NSCLC. Methods Ten patients with primary NSCLC were screened, and plasma and fecal samples were collected before and after radiotherapy, respectively. Inflammatory indices in plasma were detected. Genomic DNA was extracted from fecal specimens and sequenced on on Illumina HiSeq2000 sequencing platform. Thee sequenced data were subjected to Metagenome assembly, gene prediction, species annotation, and gene function analysis to study and analyze gut microbiota and metabolic functions. The correlation between the diversity of gut microbiota and the clinical indicators of NSCLC patients was evaluated, and the changes of gut microbiota before and after radiotherapy were observed. Results The diversity of gut microbiota in NSCLC patients did not correlate with smoking, pathology, and inflammatory markers. The abundance of phylum (p)_Bacteroidetes increased; p_Firmicutes and p_Bacteroidetes accounted for the highest proportion in NSCLC patients, and the abundance of both was dominantly exchanged after radiotherapy. There was a decrease in genus (g)_Bifidobacterium after radiotherapy in NSCLC patients. There was no significant correlation between the diversity of gut microbiota after radiotherapy and radiotherapy sensitivity, and the structural composition and abundance of gut microbiota remained stable. Conclusion The diversity of gut microbiota is altered after radiotherapy in NSCLC patients, showing an increase in harmful bacteria and a decrease in beneficial bacteria.
