Nature Communications (Feb 2017)

Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination

  • Hong Zan,
  • Connie Tat,
  • Zhifang Qiu,
  • Julia R. Taylor,
  • Justin A. Guerrero,
  • Tian Shen,
  • Paolo Casali

DOI
https://doi.org/10.1038/ncomms14244
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 16

Abstract

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Class switch DNA recombination (CSR) is critical for maturation of antibody response, and relies on Ku-mediated NHEJ of DSBs in the IgH S regions for recombination. This study shows Rad52 contributes to CSR through a Ku-independent alternative NHEJ that introduces microhomologies in S–S junctions.