Condition optimization for electroporation transfection in horse skeletal muscle satellite cells

Tseweendolmaa Ulaangerel; Minna Yi; Undarmaa Budsuren; Yingchao Shen; Hong Ren; Bold Demuul; Dongyi Bai; Dulguun Dorjgotov; Gantulga Davaakhuu; Tuyatsetseg Jambal; Manglai Dugarjav; Gerelchimeg Bou

doi:10.1080/10495398.2023.2280664

Animal Biotechnology (Dec 2024)

Condition optimization for electroporation transfection in horse skeletal muscle satellite cells

Tseweendolmaa Ulaangerel,
Minna Yi,
Undarmaa Budsuren,
Yingchao Shen,
Hong Ren,
Bold Demuul,
Dongyi Bai,
Dulguun Dorjgotov,
Gantulga Davaakhuu,
Tuyatsetseg Jambal,
Manglai Dugarjav,
Gerelchimeg Bou

Affiliations

Tseweendolmaa Ulaangerel: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Minna Yi: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Undarmaa Budsuren: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Yingchao Shen: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Hong Ren: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Bold Demuul: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Dongyi Bai: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Dulguun Dorjgotov: School of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, Mongolia
Gantulga Davaakhuu: Institute of General and Experimental Biology, Mongolian Academy of Science, Ulaanbaatar, Mongolia
Tuyatsetseg Jambal: School of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, Mongolia
Manglai Dugarjav: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Gerelchimeg Bou: lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China

DOI: https://doi.org/10.1080/10495398.2023.2280664
Journal volume & issue: Vol. 35, no. 1

Abstract

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Satellite cells are an important cellular model for studying muscle growth and development and mammalian locomotion-related molecular mechanisms. In this study, we investigated the effects of voltage, pulse duration, and DNA dosage on horse skeletal muscle satellite cells’ electroporation transfection efficiency using the eukaryotic expression plasmid Td Tomato-C1 (5.5 kb) encoding the red fluorescent protein gene mainly based on fluorescence-positive cell rate and cell survival rate. By comparison of different voltages, pulse durations, and DNA doses, horse skeletal muscle satellite cells have nearly 80% transfection efficiency under the condition of voltage 120 V, DNA dosage 7 µg/ml, and pulse duration 30 ms. This optimized electroporation condition would facilitate the application of horse skeletal muscle satellite cells in genetic studies of muscle function and related diseases.

Published in Animal Biotechnology

ISSN: 1049-5398 (Print); 1532-2378 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.tandfonline.com/journals/labt20

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