Phytoene Desaturase (PDS) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (<i>Musa</i> spp.)
Fernanda dos Santos Nascimento,
Marcelly Santana Mascarenhas,
Samantha Costa Boaventura,
Carla Catharina Hora de Souza,
Andresa Priscila de Souza Ramos,
Anelita de Jesus Rocha,
Julianna Matos da Silva Soares,
Leandro Eugenio Cardamone Diniz,
Tiago Antônio de Oliveira Mendes,
Claudia Fortes Ferreira,
Edson Perito Amorim
Affiliations
Fernanda dos Santos Nascimento
Post-Graduate Program in Biotechnology, State University of Feira de Santana, Avenida Transnordestina, Novo Horizonte, Feira de Santana 44036-900, Bahia, Brazil
Marcelly Santana Mascarenhas
Post-Graduate Program in Biotechnology, State University of Feira de Santana, Avenida Transnordestina, Novo Horizonte, Feira de Santana 44036-900, Bahia, Brazil
Samantha Costa Boaventura
Center for Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo da Bahia, Rua Rui Barbosa, 710, Centro, Cruz das Almas 44380-000, Bahia, Brazil
Carla Catharina Hora de Souza
Center for Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo da Bahia, Rua Rui Barbosa, 710, Centro, Cruz das Almas 44380-000, Bahia, Brazil
Andresa Priscila de Souza Ramos
Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas 44380-000, Bahia, Brazil
Anelita de Jesus Rocha
Post-Graduate Program in Biotechnology, State University of Feira de Santana, Avenida Transnordestina, Novo Horizonte, Feira de Santana 44036-900, Bahia, Brazil
Julianna Matos da Silva Soares
Post-Graduate Program in Biotechnology, State University of Feira de Santana, Avenida Transnordestina, Novo Horizonte, Feira de Santana 44036-900, Bahia, Brazil
Leandro Eugenio Cardamone Diniz
Embrapa Soja, Rodovia Carlos João Strass, Londrina 86085-981, Paraná, Brazil
Tiago Antônio de Oliveira Mendes
Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, Viçosa 36570-900, Minas Gerais, Brazil
Claudia Fortes Ferreira
Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas 44380-000, Bahia, Brazil
Edson Perito Amorim
Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas 44380-000, Bahia, Brazil
Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as a selective marker for genome editing in several plant species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily recognizable and highly favorable. In Musa spp., the A genome increases fruit production and quality, whereas the B genome is associated with tolerance to biotic and abiotic stresses. The objective of this study was to identify a molecular marker in the PDS gene to easily discriminate the A and B genomes of banana. A 2166 bp fragment for the “PDSMa” marker was identified as polymorphic for the A genome (identification accuracy of 99.33%), whereas ~332 and ~225 bp fragments were detected for the “PDSMb” marker with 100% accuracy using MedCalc software. In this study, we used genotypes with A and B genomes that are used in the genetic improvement of bananas and an accession with the BT genome. It was not possible to differentiate the accession with the BT genome from the others, suggesting that the markers do not have the capacity to separate the T genome from the A and B genomes. To the best of our knowledge, this is the first study to use the PDS gene to determine doses of the A genome and identify the B genome in Musa spp., which will aid in evaluating the genomic constitution of banana hybrids and accessions at the seedling stage and accelerating their classification in crop genetic improvement programs.
