Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation

Oi Wah Liew; Samantha S. M. Ling; Shera Lilyanna; Yue Zhou; Peipei Wang; Jenny P. C. Chong; Yan Xia Ng; Angeline E. S. Lim; Eliot R. Y. Leong; Qifeng Lin; Teck Kwang Lim; Qinsong Lin; Enoch M. W. Ng; Tuck Wah Ng; A. Mark Richards

doi:10.1038/s42003-021-01965-x

Communications Biology (Apr 2021)

Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation

Oi Wah Liew,
Samantha S. M. Ling,
Shera Lilyanna,
Yue Zhou,
Peipei Wang,
Jenny P. C. Chong,
Yan Xia Ng,
Angeline E. S. Lim,
Eliot R. Y. Leong,
Qifeng Lin,
Teck Kwang Lim,
Qinsong Lin,
Enoch M. W. Ng,
Tuck Wah Ng,
A. Mark Richards

Affiliations

Oi Wah Liew: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Samantha S. M. Ling: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Shera Lilyanna: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Yue Zhou: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Peipei Wang: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Jenny P. C. Chong: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Yan Xia Ng: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Angeline E. S. Lim: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Eliot R. Y. Leong: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System
Qifeng Lin: Department of Biological Sciences, National University of Singapore
Teck Kwang Lim: Department of Biological Sciences, National University of Singapore
Qinsong Lin: Department of Biological Sciences, National University of Singapore
Enoch M. W. Ng: Laboratory for Optics & Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University
Tuck Wah Ng: Laboratory for Optics & Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University
A. Mark Richards: Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System

DOI: https://doi.org/10.1038/s42003-021-01965-x
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 17

Abstract

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Liew et al. describe an efficient monoclonal antibody (mAb) production method and as a proof-of-concept, develop mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for antibody panel generation.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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