Nature Communications (Mar 2023)
CRISPR-induced DNA reorganization for multiplexed nucleic acid detection
- Margot Karlikow,
- Evan Amalfitano,
- Xiaolong Yang,
- Jennifer Doucet,
- Abigail Chapman,
- Peivand Sadat Mousavi,
- Paige Homme,
- Polina Sutyrina,
- Winston Chan,
- Sofia Lemak,
- Alexander F. Yakunin,
- Adam G. Dolezal,
- Shana Kelley,
- Leonard J. Foster,
- Brock A. Harpur,
- Keith Pardee
Affiliations
- Margot Karlikow
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Evan Amalfitano
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Xiaolong Yang
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Jennifer Doucet
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Abigail Chapman
- Department of Biochemistry & Molecular Biology, Michael Smith Laboratories, University of British Columbia
- Peivand Sadat Mousavi
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Paige Homme
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Polina Sutyrina
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Winston Chan
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Sofia Lemak
- Department of Chemical Engineering and Applied Chemistry, University of Toronto
- Alexander F. Yakunin
- Department of Chemical Engineering and Applied Chemistry, University of Toronto
- Adam G. Dolezal
- Department of Entomology, University of Illinois at Urbana–Champaign
- Shana Kelley
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- Leonard J. Foster
- Department of Biochemistry & Molecular Biology, Michael Smith Laboratories, University of British Columbia
- Brock A. Harpur
- Department of Entomology, Purdue University
- Keith Pardee
- Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto
- DOI
- https://doi.org/10.1038/s41467-023-36874-6
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 11
Abstract
Nucleic acid sensing involving CRISPR technologies is powerful but has certain limitations, such as PAM sequence requirements and limited multiplexing. Here, authors report a CRISPR-based barcoding technology which enables multiple outputs from any target sequence, based on cis- and trans-cleavage.
