Effects of chronic nitrite exposure on hematological parameters, oxidative stress and apoptosis in spotted seabass (Lateolabrax maculatus) reared at high temperature

Chen Shen; Shi Cao; Mohamed Mohsen; Xue-Shan Li; Ling Wang; Kang-Le Lu; Chun-Xiao Zhang; Kai Song

Aquaculture Reports (Apr 2024)

Effects of chronic nitrite exposure on hematological parameters, oxidative stress and apoptosis in spotted seabass (Lateolabrax maculatus) reared at high temperature

Chen Shen,
Shi Cao,
Mohamed Mohsen,
Xue-Shan Li,
Ling Wang,
Kang-Le Lu,
Chun-Xiao Zhang,
Kai Song

Affiliations

Chen Shen: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Fisheries College, Jimei University, Xiamen 361021, China
Shi Cao: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Fisheries College, Jimei University, Xiamen 361021, China
Mohamed Mohsen: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Fisheries College, Jimei University, Xiamen 361021, China
Xue-Shan Li: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Fisheries College, Jimei University, Xiamen 361021, China
Ling Wang: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Fisheries College, Jimei University, Xiamen 361021, China
Kang-Le Lu: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Fisheries College, Jimei University, Xiamen 361021, China
Chun-Xiao Zhang: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Fisheries College, Jimei University, Xiamen 361021, China
Kai Song: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Fisheries College, Jimei University, Xiamen 361021, China; Corresponding author at: State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China.

Journal volume & issue: Vol. 35
p. 102022

Abstract

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The effects of nitrite stress on blood biochemistry, antioxidant capacity, and apoptosis of spotted seabass (Lateolabrax maculatus) under high-temperature conditions were investigated. Fish were subjected to varying levels of nitrite concentrations for 28 days, specifically 0, 8, and 16 mg/L. Blood and gill samples were collected at the 1st, 3rd, 7th, 14th, 21st and 28th of the exposure for index determination, respectively. Results showed that as the concentration of nitrite increased, blood hemoglobin (Hb) and serum chloride ion (Cl-) levels decreased. In addition, compared to the control group, methemoglobin (MetHb), hematocrit (HCT), red blood cells (RBCs), white blood cells (WBCs), and serum potassium ion (K+) were significantly increased in the nitrite-stressed group. In terms of antioxidants, catalase (CAT) and superoxide dismutase (SOD) activities in the gill were significantly decreased with increasing nitrite concentration. Additionally, malondialdehyde (MDA) content was significantly increased with increasing nitrite concentration. Regarding genes related to apoptosis, as nitrite concentration increased, the expressions of hypoxia-inducible factor (Hif-1α) and cytochrome C (Cyt-c) genes in the gill were significantly up-regulated on the 1st and 3rd day of exposure. The expressions of P53, Bax, Caspase-3, Caspase-8, and Caspase-9 genes in the gill were significantly up-regulated in stress groups at different exposure time intervals compared to the control group. In summary, nitrite caused disruption in the balance of blood ions, decreased antioxidant capacity in the gills, increased expression of genes associated with apoptosis, ultimately resulting in gill injury in the spotted seabass.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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