Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3Δ25bp variant

Prasanth Chimata; Deepak K Kashyap; Thiagarajan Sairam; Akshayaa Ganesh; Kumarasamy Thangaraj; Meera Purushottam; Biju Viswanath; Sanjeev Jain; Perundurai S Dhandapany

Stem Cell Research (Dec 2022)

Generation of a new human induced pluripotent stem cell (hiPSC) line from a South Asian Indian with a MYBPC3Δ25bp variant

Prasanth Chimata,
Deepak K Kashyap,
Thiagarajan Sairam,
Akshayaa Ganesh,
Kumarasamy Thangaraj,
Meera Purushottam,
Biju Viswanath,
Sanjeev Jain,
Perundurai S Dhandapany

Affiliations

Prasanth Chimata: Cardiovascular Biology and Disease Theme, Institute for Stem Cell Science and Regenerative Medicine, Bangalore (inStem), Bangalore, India; Manipal Academy of Higher Education, Manipal, India
Deepak K Kashyap: Cardiovascular Biology and Disease Theme, Institute for Stem Cell Science and Regenerative Medicine, Bangalore (inStem), Bangalore, India; CSIR-Center for Cellular and Molecular Biology (CCMB), Hyderabad, India
Thiagarajan Sairam: Cardiovascular Biology and Disease Theme, Institute for Stem Cell Science and Regenerative Medicine, Bangalore (inStem), Bangalore, India
Akshayaa Ganesh: Molecular Genetics Laboratory, Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bengaluru, India
Kumarasamy Thangaraj: CSIR-Center for Cellular and Molecular Biology (CCMB), Hyderabad, India; Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad, India
Meera Purushottam: Molecular Genetics Laboratory, Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bengaluru, India
Biju Viswanath: Molecular Genetics Laboratory, Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bengaluru, India
Sanjeev Jain: Molecular Genetics Laboratory, Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bengaluru, India
Perundurai S Dhandapany: Cardiovascular Biology and Disease Theme, Institute for Stem Cell Science and Regenerative Medicine, Bangalore (inStem), Bangalore, India; Corresponding author.

Journal volume & issue: Vol. 65
p. 102978

Abstract

Read online

Myosin binding protein C3 (MYBPC3) is a thick filament contractile protein that interacts with myosin, titin and actin and regulates cardiac muscle contraction. Genetic variations in the MYBPC3 gene are known causal factors for cardiomyopathy and heart failure. Previously, we identified a recurrent MYBPC3 deletion (25 base pairs) among South Asians associated with cardiomyopathy and heart failure. Here, we generated an induced pluripotent stem cell (iPSC) line using peripheral blood mononuclear cells (PBMC) from an Indian harboring MYBPC3 deletion. This iPSC line displayed embryonic stem cell morphology, expressed pluripotency markers, differentiated into three germ layers and exhibited normal karyotype.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal