Effects of matrix metalloproteinase inhibition in cultured human Tenon’s capsule fibroblasts

Abstract

Abstract Background This study aimed to compare the characteristics of anterior and posterior human Tenon’s capsule fibroblasts and determine the effect of matrix metalloproteinase (MMP) inhibition on cultured human Tenon’s capsule fibroblasts. Methods Tissues obtained from the anterior and posterior Tenon’s capsules were partially incised, cultured, and subjected to microarray analysis. Electrophoresis was performed on the cells treated with Ilomastat (MMP-9 inhibitor), and collagen 1, 3, and 6. Antibodies against transforming growth factor-beta (TGF-β) and vimentin were used as probes to compare the expression levels. Results There were no differences between anterior and posterior Tenon’s capsule fibroblasts in the microarray. Western blot analysis showed similar expression of collagen 1, 3, and 6, TGF-β, and vimentin between anterior and posterior Tenon’s capsule fibroblast. Ilomastat did not affect the proliferation of cultured human Tenon's capsule. Conclusions No differences in characteristics were observed between anterior and posterior Tenon’s capsule fibroblasts, and inhibition of MMPs did not affect the proliferation of cultured human Tenon’s capsule fibroblasts.

Keywords

• Antimetabolite
• Tenon’s capsule fibroblast
• Matrix metalloproteinase