Evaluation of Bone Morphogenetic Protein-4 gene polymorphism for growth traits in Indian goat breeds

Abstract

The genetic improvement of production traits can be made through marker assisted selection using a candidate gene approach. Bone morphogenetic proteins (BMPs) are multifunctional growth factors that belong to the transforming growth factor b (TGF-b) superfamily. This study aimed to detect the genetic polymorphism of BMP-4 in different goat breeds by polymerase chain reaction-single strand confirmational polymorphism (PCR-SSCP) and association of polymorphic variants with growth traits. The amplified fragments of BMP4 gene of 380 bp size were analysed using SSCP in non-denaturing PAGE and the results showed the presence of two genotypes: AA (47- 67%) and BB (33-53%) in Barbari, Sirohi and Black Bengal breed. The association of BMP-4 polymorphism with different growth trait parameters showed non-significant effect of genotypes. However, some genotypes showed non-significant superiority over others. Further research on a large population is required to validate the role of the BMP-4 gene in goat growth traits.

Keywords

• BMP-4, Goat, Growth traits, PCR-SSCP