Changes in Expression of Proteolytic-Related Genes in Chick Myoblasts during Myogenesis

Abstract

Changes in expression of proteolytic-related genes in chick myoblasts during myogenesis were investigated. Expression of MyoD and myogenin, as an index of differentiation, was regulated in chick myoblasts during myogenesis. Expression of PAX7, as an index of myoblast proliferation, was decreased during myogenesis. Expression of ubiquitin, atrogin-1/MAFbx, proteasome C2 subunit, m-calpain large subunit, cathepsin B, and caspase-3 mRNA of chick myoblasts was also decreased during myogenesis. These results show that the expression of proteolytic-related genes of myoblasts was decreased during myogenesis, resulting in an increase in differentiation of chick myoblasts.

Keywords

• atrogin-1/mafbx
• myoblasts
• myogenesis
• differentiation
• chicken