Nature Communications (May 2020)

A genome-scale map of DNA methylation turnover identifies site-specific dependencies of DNMT and TET activity

  • Paul Adrian Ginno,
  • Dimos Gaidatzis,
  • Angelika Feldmann,
  • Leslie Hoerner,
  • Dilek Imanci,
  • Lukas Burger,
  • Frederic Zilbermann,
  • Antoine H. F. M. Peters,
  • Frank Edenhofer,
  • Sébastien A. Smallwood,
  • Arnaud R. Krebs,
  • Dirk Schübeler

DOI
https://doi.org/10.1038/s41467-020-16354-x
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 16

Abstract

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Local activity of the DNA methylation machinery remains poorly understood. Here, the authors present a theoretical and experimental framework to infer methylation and demethylation rates at genome scale in mouse embryonic stem cells, finding that maintenance methylation activity is reduced at transcription factor binding sites, while methylation turnover is elevated in transcribed gene bodies.