Influence of a macroporous β-TCP structure on human mesenchymal stem cell proliferation and differentiation in vitro
Shaan Chamary,
Liliana Grenho,
Maria Helena Fernandes,
Franck Bouchart,
Fernando Jorge Monteiro,
Jean Christophe Hornez
Affiliations
Shaan Chamary
Université Polytechnique Hauts-de-France, EA 2443 - Laboratoire des Matériaux Céramiques et Procédés Associés, Boulevard Charles de Gaulle, 59600, Maubeuge, France; Corresponding author.
Liliana Grenho
Bone Lab, Faculty of Dental Medicine, U. Porto, Rua Dr. Manuel Pereira da Silva 4200-393, Porto, Portugal
Maria Helena Fernandes
Bone Lab, Faculty of Dental Medicine, U. Porto, Rua Dr. Manuel Pereira da Silva 4200-393, Porto, Portugal
Franck Bouchart
Université Polytechnique Hauts-de-France, EA 2443 - Laboratoire des Matériaux Céramiques et Procédés Associés, Boulevard Charles de Gaulle, 59600, Maubeuge, France
Fernando Jorge Monteiro
Instituto de Engenharia Biomédica, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; Universidade Do Porto, Faculdade de Engenharia, Departamento de Engenharia Metalúrgica e de Materiais, Rua Dr.Roberto Frias S/n 4200-465, Porto, Portugal; I3S- Instituto de Investigação e Inovação Em Saúde, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal
Jean Christophe Hornez
Université Polytechnique Hauts-de-France, EA 2443 - Laboratoire des Matériaux Céramiques et Procédés Associés, Boulevard Charles de Gaulle, 59600, Maubeuge, France
This work aims at studying the links between a porous scaffold in β-TCP and its impact on cell differentiation and proliferation in vitro. β-TCP was synthesized by aqueous precipitation. The shaping methods were chosen for their ability to generate original structures with the potential to positively influence cell behaviour. The impregnation of polymeric structure (PS) yielded an interconnected network of spherical pores, stereolithography (3D) yielded a network of interconnected diamond shaped pores and freeze casting (FC) yielded a network of parallel ellipsoidal channel-like pores. Two different trends emerged from the human mesenchymal stem cell culture: 3D and PS seems to promote surface proliferation whereas the freeze casted samples promoted cell penetration, bulk colonization, expression of key osteoblastic genes (RunX-2, ALP, BMP-2, BGLAP and Collagen) and alkaline phosphatase activity. The architectural features created by freeze casting positively influenced human mesenchymal stem cells behaviour in vitro.
