Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis

Abstract

Phytase has a hydrolysis function on phytic acid which yields inorganic phosphate. Using phytase enzymes in domestic animals have a positive effect on optimal diet intake. Bacillus species can only produce thermostable alkaline phytase. Accordingly extracellular phytase gene was isolated from Bacillus subtilis ATCC 12711 (phyc) using linker primers containing restriction sites of BamHI and HindIII. The isolated fragment was then inserted into pTZ57R/T vector for cloning and nucleotide characterization. Molecular weight of phytase protein was estimated about 42 kDa. On the other hand the model of phytase secondary structure showed the potential of thermostable characterization of enzyme

Keywords

• phytase enzyme
• gene cloning
• bacillus subtilis
• phytic acide
• recombinant protein