Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA

Aravind Natarajan; Alvin Han; Soumaya Zlitni; Erin F. Brooks; Summer E. Vance; Marlene Wolfe; Upinder Singh; Prasanna Jagannathan; Benjamin A. Pinsky; Alexandria Boehm; Ami S. Bhatt

doi:10.1038/s41467-021-25576-6

Nature Communications (Oct 2021)

Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA

Aravind Natarajan,
Alvin Han,
Soumaya Zlitni,
Erin F. Brooks,
Summer E. Vance,
Marlene Wolfe,
Upinder Singh,
Prasanna Jagannathan,
Benjamin A. Pinsky,
Alexandria Boehm,
Ami S. Bhatt

Affiliations

Aravind Natarajan: Department of Genetics, Stanford University
Alvin Han: Department of Microbiology and Immunology, Stanford University
Soumaya Zlitni: Department of Genetics, Stanford University
Erin F. Brooks: Department of Medicine (Hematology, Blood and Marrow Transplantation), Stanford University
Summer E. Vance: Department of Medicine (Hematology, Blood and Marrow Transplantation), Stanford University
Marlene Wolfe: Department of Civil and Environmental Engineering, Stanford University
Upinder Singh: Department of Medicine (Infectious Diseases and Geographic Medicine), Stanford University
Prasanna Jagannathan: Department of Microbiology and Immunology, Stanford University
Benjamin A. Pinsky: Department of Medicine (Infectious Diseases and Geographic Medicine), Stanford University
Alexandria Boehm: Department of Civil and Environmental Engineering, Stanford University
Ami S. Bhatt: Department of Genetics, Stanford University

DOI: https://doi.org/10.1038/s41467-021-25576-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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While the analysis of SARS-CoV-2 RNA in stool samples has led to important insights regarding the disease, quantification is currently challenging. Here the authors use patient samples to benchmark preservation, extraction and quantification methods to optimise detection of viral RNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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