SNP identification and validation in two invasive species: zebra mussel (Dreissena polymorpha) and Asian clam (Corbicula fluminea)

Abstract

The development of affordable massive parallel sequencing (MPS) has reduced both time and costs of SNP identification for use in conservation and population genetic studies. After MPS, a second validation is usually required. High resolution melting analysis (HRMA) is a fast and simple method for mutation scanning, and thus a suitable validation protocol, particularly in non–model species. We present a set of nine novel polymorphic SNPs identified by MPS and validated with HRMA in two invasive species (the zebra mussel Dreissena polymorpha and the Asian clam Corbicula fluminea). These SNPs can be used in genetic studies to accurately assess and understand past and future invasion events.

Keywords

• corbicula fluminea
• dreissena polymorpha
• invasive species
• massive parallel sequencing
• single nucleotide polymorphisms