Preliminary Assessment of the Protective and Antitumor Effects of Several Phytoene-Containing Bacterial and Microalgal Extracts in Colorectal Cancer
Gloria Perazzoli,
Cristina Luque,
Antonio León-Vaz,
Patricia Gómez-Villegas,
Rocío Rengel,
Ana Molina-Márquez,
Ángeles Morón-Ortiz,
Paula Mapelli-Brahm,
José Prados,
Consolación Melguizo,
Antonio Meléndez-Martínez,
Rosa León
Affiliations
Gloria Perazzoli
Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), 18100 Granada, Spain
Cristina Luque
Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), 18100 Granada, Spain
Antonio León-Vaz
Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, Spain
Patricia Gómez-Villegas
Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, Spain
Rocío Rengel
Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, Spain
Ana Molina-Márquez
Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, Spain
Ángeles Morón-Ortiz
Food Color and Quality Laboratory, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
Paula Mapelli-Brahm
Food Color and Quality Laboratory, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
José Prados
Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), 18100 Granada, Spain
Consolación Melguizo
Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), 18100 Granada, Spain
Antonio Meléndez-Martínez
Food Color and Quality Laboratory, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
Rosa León
Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, Spain
The identification of new functional food constituents is a priority to improve the prognosis and prevention of colorectal cancer (CRC). In this study, several bacterial and algal phytoene-enriched extracts were obtained, and their potential activity against oxidative damage and their ability to inhibit proliferation and cell migration in several human colon-adenocarcinoma-derived cell lines were assessed. The main conclusions indicate that total extracts of Sphingomonas echinoides and Chlorella sorokiniana exhibited the highest protective effect against oxidative damage. All extracts enhanced the activity of detoxifying enzymes, particularly importantly the increase of NAD(P)H:quinone oxidoreductase activity, which reached a value 40% higher than that of untreated control cells upon exposure to Escherichia coli extracts. Staphylococcus haemolyticus and transgenic E. coli extracts significantly arrested the migration capacity of both cell lines, while S. haemolyticus and C. sorokiniana extracts inhibited cell proliferation by 15 to 20% compared to untreated cells. These results point to these extracts as potential antioxidant complements able to protect cells against oxidative damage and with a moderate ability to inhibit the proliferation and migration of CRC tumor cells, paving the way to design functional foods or probiotic formulations with preventive properties against oxidative stress-related diseases, such as cancer, or as starting point for purifying anticancer compounds.
