Zhejiang Daxue xuebao. Lixue ban (May 2009)

Intra specific DNA sequence polymorphism and genetic markers in the mitochon drial COII gene from five color patterns of Oujiang color carp(5种体色瓯江彩鲤线粒体COII基因的序列差异和遗传标记研究)

  • LVYao-ping(吕耀平),
  • HUZe-hui(胡则辉),
  • XIAOJian-zhong(肖建中),
  • WANGCheng-hui(王成辉),
  • HUJin-qiang(胡金强),
  • LAOShen-ying(劳沈颖),
  • YELi-ping(叶丽平),
  • XIANGSong-ping(项松平),
  • LIUQing-e(刘青娥)

DOI
https://doi.org/10.3785/j.issn.1008-9497.2009.03.018
Journal volume & issue
Vol. 36, no. 3
pp. 323 – 327

Abstract

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利用PCR方法扩增获得瓯江彩鲤(Cyprinus carpio var. color)线粒体DNA的COII基因,测定该基因片段序列.分析了瓯江彩鲤“全红”、“粉玉”、“粉花”、“麻花”和“大花”共20只个体的序列核苷酸位点差异和遗传多态性.结果表明,“大花”和“全红”遗传多样性最为丰富,“粉花”的遗传多样性相对贫乏.在长度为604 bp的基因片段中,共检测到9个多态性核苷酸位点（占1.49%) ,20只个体具有4种基因型,5种体色各自的平均核苷酸位点差异分别为0.38%、0.2%、0、0.13%和0.47%. UPGMA分子系统聚类树显示,“粉花”、“麻花”和“粉玉”的遗传关系接近；“全红”相对独立成一支,与其他4种体色瓯江彩鲤的遗传关系较远.COII基因可以作为区分三分支群体的遗传标记.

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