Isolation, identification of entomopathogenic nematodes with insights into their distribution in the Syrian coast regions and virulence against Tuta absoluta

Abstract

The occurrence and distribution of entomopathogenic nematodes (EPNs) in the Syrian coast regions remain relatively uncharted. To address this gap in our knowledge, an extensive survey of these ecosystems was essential. This study aims to isolate and identify EPNs from diverse ecosystems within the coastal regions. The distribution of EPNs in cultivated and natural environments was analyzed according to habitat, altitude, and sampling season factors. Between 2017 and 2020, EPNs were recovered from 27 out of 821 soil samples (3.28%) and collected from 24 out of 375 sampling sites (6.4%). Based on morphological, morphometric, and molecular (ITS) characteristics, four EPN species were identified: Heterorhabditis indica (51.85%), representing the first report of its occurrence in the coastal regions, H. bacteriophora (33.33%), H. pakistanense (7.4%), which is also reported for the first time in Syria, and Steinernema affine (7.4%). There were statistical differences in the abundance and recovery frequency of EPNs in each type of habitat. Additionally, there were statistical differences in the altitude and sampling season recovery frequency.

Keywords

• heterorhabditis
• steinernema
• identification
• distribution
• biological control
• syria