Majallah-i ̒Ilmī-i Shīlāt-i Īrān (Jan 2019)
Evaluation of the effects of diethyl ether and methanol extracts of carpet anemone (Sarcophyton spp.) and starfish (Pentaceraster spp.) on E. coli and Bacillus subtilis bacteria
Abstract
Marine environment is the main source of natural bioactive products whose chemical-structural properties are not similar with other natural products of plants and other inland organisms. The present study was carried out to evaluate antibiotic properties of diethyl ether and methanol extracts of two marine species including starfish and carpet anemone. The samples of starfish and carpet anemone were collected from the Lark Island coasts. Extraction was done using diethyl ether and methanol solvents and antibiotic properties analysis on Escherichia coli and Bacillus subtilis were evaluated using agar diffusion method. Then tube dilution to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). According to the results, diethyl ether extract from carpet anemone had no inhibitory effect on E. coli tested bacteria. While, starfish methanol extract(40 mg/ml) and hadshowed a growth inhibitory effect on E. coli bacteriastarfish at a concentration of 40 mg/ml for E. coli bacteria. [M1] [32] The starfish methanol extract from starfish at concentration of (30 and 50 30 mg/ml) had an growth inhibitory effect onfor B. subtilis bacteria had an inhibitory effect on growth and at concentration of 50 mg/ml. The effect of Carpet anemone methanolic extract of (carpet anemone at 40 mg/ml) cause a growth inhibitory effect on for E. coli had an inhibitory effect on growth, but did not have a deleterious effect. starfish methanol extract [M3] [34] showed the highest growth inhibitory effect onThe diameter of the inhibition zone for B. subtilis and created a growth inhibitory circle zoonthe methanolic extract of the starfish showed of ( 12.1±0.8 mm diameter). [M5] [36] The results showed that the methanolic extract of the starfish has higher growth inhibitory effect on the two studied bacterial species. In conclusion, methanolic extracts of the starfish were effective in inhibiting the growth of Bacillus subtilis bacteria and methanolic extract of carpet anemone in inhibiting E. coli bacteria growth. methanolic extracts of the starfish and carpet anemone were effective in inhibiting the growth of B. subtilis and E. coli bacteria, respectively.[M7] [38]
