International Journal of Women's Health (Feb 2022)
Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese Women
Abstract
Xi-Yuan Jiang,1,2 Qing Wang,1 Ying Zhang,3 Yong Chen,1 Long-Fei Wu4 1Center of Osteoporosis, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, Jiangsu, 215300, People’s Republic of China; 2School of Medicine, Southeast University, Nanjing, Jiangsu, 210009, People’s Republic of China; 3Department of Clinical Laboratory, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, Jiangsu, 215300, People’s Republic of China; 4School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu, 215123, People’s Republic of ChinaCorrespondence: Xi-Yuan JiangCenter of Osteoporosis, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, Jiangsu, 215300, People’s Republic of China, Tel/Fax +86-512-57928140, Email [email protected]: Chemerin has been suggested to be a risk factor for osteoporosis; however, its relationship with osteoporotic fracture is poorly understood. Herein, we intend to explore the association between serum chemerin and osteoporotic fracture.Methods: A total of 111 elderly women patients diagnosed with osteoporotic fracture were selected as the observation group, and 40 healthy subjects were enrolled as controls. Dual-energy X-ray absorptiometry, enzyme-linked immunosorbent assay, electrochemiluminescence immunoassay, and biochemical analysis were separately performed to determine body bone mineral density (BMD), chemerin levels, bone turnover markers, and other parameters. Pearson’s correlation analysis was conducted to examine a relationship between chemerin and laboratory parameters. Moreover, the levels of chemokine-like receptor 1 (CMKLR), C-C motif chemokine receptor-like 2 (CCRL2), collagen type I alpha (COLA1), and runt-related transcription factor-2 (RUNX2) were confirmed by quantitative polymerase chain reaction, and the effect of chemerin on osteogenic differentiation of hFOB1.19 cells was indicated by tartrate-resistant acid phosphatase and alkaline phosphatase double staining.Results: A higher level of chemerin was generally detected in patients with osteoporotic fracture compared with those without (P 0.05), whereas there was a remarkable decrease of COLA1 and RUNX2 after incubation with chemerin for nine days (all P< 0.05). Furthermore, prolonged incubation with chemerin enhanced osteoclast differentiation and maturation, consequently contributing to an increased risk of fracture.Conclusion: Chemerin is a strong and independent risk factor for osteoporosis-related fracture among elderly Chinese women.Keywords: chemerin, osteoporotic fracture, risk factor, elderly women
