Study of the Metabolite Changes in <i>Ganoderma lucidum</i> under Pineapple Leaf Residue Stress via LC-MS/MS Coupled with a Non-Targeted Metabolomics Approach
Yijun Liu,
Yangyang Qian,
Chunyu Wang,
Yingying He,
Chuxing Zhu,
Gang Chen,
Lijing Lin,
Yuliang Chen
Affiliations
Yijun Liu
State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China
Yangyang Qian
State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China
Chunyu Wang
State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China
Yingying He
State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China
Chuxing Zhu
State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China
Gang Chen
State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China
Lijing Lin
Hainan Key Laboratory of Storage & Processing of Fruits and Vegetables, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, China
Yuliang Chen
Hainan Key Laboratory of Storage & Processing of Fruits and Vegetables, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, China
The effects of fermentation metabolites of G. lucidum under different pineapple leaf residue additions were separated and identified using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The mass spectra showed that the metabolites had good response values only in the positive ion mode, and 3019 metabolites with significant differences, mainly distributed in 95 metabolic pathways, were identified. The multivariate analyses, including the principal component analysis (PCA), orthogonal least squares discriminant analysis (OPLS-DA), and volcano plots (VP), revealed that the G. lucidum metabolites exhibited significant differences (p G. lucidum and improving its utilization rate and added value.
