Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
Wei-Hung Lin,
Yen-Zhen Zhang,
Po-Yao Liu,
Po-Shun Chen,
Shining Wang,
Pei-Yun Kuo,
Tran Thi Dieu Thuy,
Tran Thi Thuy Duong,
Li-Li Wen,
Yi-Hsien Hsieh,
Ming-Cheng Wang,
Cheng-Yen Kao
Affiliations
Wei-Hung Lin
Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
Yen-Zhen Zhang
Institute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Po-Yao Liu
Institute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Po-Shun Chen
Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089-0482, USA
Shining Wang
Institute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Pei-Yun Kuo
Institute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Tran Thi Dieu Thuy
Institute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Tran Thi Thuy Duong
Institute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Li-Li Wen
Department of Clinical Laboratory, En Chu Kong Hospital, New Taipei City 237, Taiwan
Yi-Hsien Hsieh
Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan
Ming-Cheng Wang
Division of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
Cheng-Yen Kao
Institute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Escherichia coli causing urinary tract infections (UTIs) are one of the most common outpatient bacterial infections. This study aimed to compare the characteristics of E. coli isolated from UTI patients in a single medical center in 2009–2010 (n = 504) and 2020 (n = 340). The antimicrobial susceptibility of E. coli was determined by the disk diffusion method. PCRs were conducted to detect phylogenetic groups, ST131, K1 capsule antigen, and 15 virulence factors. Phylogenetic group B2 dominated in our 2009–2010 and 2020 isolates. Moreover, no phylogenetic group E strains were isolated in 2020. E. coli isolates in 2020 were more susceptible to amoxicillin, ampicillin/sulbactam, cefuroxime, cefmetazole, ceftazidime, cefoxitin, tetracycline, and sulfamethoxazole/trimethoprim, compared to the isolates in 2009–2010. Extensively drug-resistant (XDR)-E. coli in 2009–2010 were detected in groups B1 (5 isolates), B2 (12 isolates), F (8 isolates), and unknown (1 isolate). In 2020, XDR-E. coli were only detected in groups A (2 isolates), B2 (5 isolates), D (1 isolate), and F (4 isolates). The prevalence of virulence factor genes aer and fimH were higher in E. coli in 2009–2010 compared to those in 2020. In contrast, afa and sat showed higher frequencies in E. coli isolates in 2020 compared to E. coli in 2009–2010.
