Dietary Rice Bran-Modified Human Gut Microbial Consortia Confers Protection against Colon Carcinogenesis Following Fecal Transfaunation
Kristopher D. Parker,
Akhilendra K. Maurya,
Hend Ibrahim,
Sangeeta Rao,
Petronella R. Hove,
Dileep Kumar,
Rama Kant,
Bupinder Raina,
Rajesh Agarwal,
Kristine A. Kuhn,
Komal Raina,
Elizabeth P. Ryan
Affiliations
Kristopher D. Parker
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA
Akhilendra K. Maurya
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
Hend Ibrahim
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA
Sangeeta Rao
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA
Petronella R. Hove
Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO 80523, USA
Dileep Kumar
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
Rama Kant
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
Bupinder Raina
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
Rajesh Agarwal
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
Kristine A. Kuhn
Division of Rheumatology, Department of Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USA
Komal Raina
Department of Pharmaceutical Sciences, South Dakota State University, Brookings, SD 57007, USA
Elizabeth P. Ryan
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA
Rice bran, removed from whole grain rice for white rice milling, has demonstrated efficacy for the control and suppression of colitis and colon cancer in multiple animal models. Dietary rice bran intake was shown to modify human stool metabolites as a result of modifications to metabolism by gut microbiota. In this study, human stool microbiota from colorectal cancer (CRC) survivors that consumed rice bran daily was examined by fecal microbiota transplantation (FMT) for protection from azoxymethane and dextran sodium sulfate (AOM/DSS) induced colon carcinogenesis in germ-free mice. Mice transfaunated with rice bran-modified microbiota communities (RMC) harbored fewer neoplastic lesions in the colon and displayed distinct enrichment of Flavonifractor and Oscillibacter associated with colon health, and the depletion of Parabacteroides distasonis correlated with increased tumor burden. Two anti-cancer metabolites, myristoylcarnitine and palmitoylcarnitine were increased in the colon of RMC transplanted mice. Trimethylamine-N-oxide (TMAO) and tartarate that are implicated in CRC development were reduced in murine colon tissue after FMT with rice bran-modified human microbiota. Findings from this study show that rice bran modified gut microbiota from humans confers protection from colon carcinogenesis in mice and suggests integrated dietary-FMT intervention strategies should be tested for colorectal cancer control, treatment, and prevention.
