A method for screening tigecycline-resistant gene tet(X) from human gut

Yu Zeng; Jiayue Lu; Congcong Liu; Zhuoren Ling; Qiaoling Sun; Hanyu Wang; Hongwei Zhou; Yanyan Hu; Gongxiang Chen; Rong Zhang

Journal of Global Antimicrobial Resistance (Mar 2021)

A method for screening tigecycline-resistant gene tet(X) from human gut

Yu Zeng,
Jiayue Lu,
Congcong Liu,
Zhuoren Ling,
Qiaoling Sun,
Hanyu Wang,
Hongwei Zhou,
Yanyan Hu,
Gongxiang Chen,
Rong Zhang

Affiliations

Yu Zeng: Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
Jiayue Lu: Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
Congcong Liu: Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
Zhuoren Ling: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
Qiaoling Sun: Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
Hanyu Wang: Liberal Art and Science, University of Connecticut, CT, USA
Hongwei Zhou: Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
Yanyan Hu: Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
Gongxiang Chen: Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China; Corresponding authors.
Rong Zhang: Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China; Corresponding authors.

Journal volume & issue: Vol. 24
pp. 29 – 31

Abstract

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Objectives: To develop an effective enrichment method for tet(X) detection, we performed PCR and Sanger sequencing to screen and confirm the presence of tet(X) gene. Methods: Species were identified by MALDI-TOF MS analysis. The minimum inhibitory concentrations (MICs) of common antibiotics were determined by broth microdilution and interpreted according to the CLSI guidelines and EUCAST breakpoints. Results: We obtained 29 (2.26%, 29/1284) tet(X4)-positive Escherichia coli, and 96.6% of those (28 isolates) exhibited resistance to tigecycline. Conclusion: This specific screening strategy for functional tet(X) mediating tigecycline resistance will be useful to facilitate development and advancement of our knowledge of tet(X).

Published in Journal of Global Antimicrobial Resistance

ISSN: 2213-7165 (Print); 2213-7173 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Microbiology
Website: https://www.journals.elsevier.com/journal-of-global-antimicrobial-resistance

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