Nature Communications (Dec 2020)
Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysis
- Rowena A. Bull,
- Thiruni N. Adikari,
- James M. Ferguson,
- Jillian M. Hammond,
- Igor Stevanovski,
- Alicia G. Beukers,
- Zin Naing,
- Malinna Yeang,
- Andrey Verich,
- Hasindu Gamaarachchi,
- Ki Wook Kim,
- Fabio Luciani,
- Sacha Stelzer-Braid,
- John-Sebastian Eden,
- William D. Rawlinson,
- Sebastiaan J. van Hal,
- Ira W. Deveson
Affiliations
- Rowena A. Bull
- The Kirby Institute for Infection and Immunity, University of New South Wales
- Thiruni N. Adikari
- The Kirby Institute for Infection and Immunity, University of New South Wales
- James M. Ferguson
- Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research
- Jillian M. Hammond
- Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research
- Igor Stevanovski
- Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research
- Alicia G. Beukers
- NSW Health Pathology, Department of Infectious Diseases and Microbiology, Royal Prince Alfred Hospital
- Zin Naing
- School of Medical Sciences, Faculty of Medicine, University of New South Wales
- Malinna Yeang
- School of Medical Sciences, Faculty of Medicine, University of New South Wales
- Andrey Verich
- The Kirby Institute for Infection and Immunity, University of New South Wales
- Hasindu Gamaarachchi
- Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research
- Ki Wook Kim
- Virology Research Laboratory, Serology and Virology Division (SAViD), NSW Health Pathology, Prince of Wales Hospital
- Fabio Luciani
- The Kirby Institute for Infection and Immunity, University of New South Wales
- Sacha Stelzer-Braid
- School of Medical Sciences, Faculty of Medicine, University of New South Wales
- John-Sebastian Eden
- Marie Bashir Institute for Infectious Diseases and Biosecurity & Sydney Medical School, The University of Sydney
- William D. Rawlinson
- School of Medical Sciences, Faculty of Medicine, University of New South Wales
- Sebastiaan J. van Hal
- NSW Health Pathology, Department of Infectious Diseases and Microbiology, Royal Prince Alfred Hospital
- Ira W. Deveson
- Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research
- DOI
- https://doi.org/10.1038/s41467-020-20075-6
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 8
Abstract
Nanopore sequencing (ONT) has been used in SARS-CoV-2 studies, however adoption of ONT for SARS-CoV-2 surveillance has been limited due to common concerns around sequencing accuracy. Here, the authors perform a comprehensive evaluation of ONT analytical performance on 157 matched SARS-CoV-2-positive patient specimens and synthetic RNA controls.
