Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa
Barbara Kij-Mitka,
Halina Cernohorska,
Svatava Kubickova,
Sylwia Prochowska,
Wojciech Niżański,
Joanna Kochan,
Monika Bugno-Poniewierska
Affiliations
Barbara Kij-Mitka
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture, Mickiewicza 24/28, 30-059 Krakow, Poland
Halina Cernohorska
Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
Svatava Kubickova
Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
Sylwia Prochowska
Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Grunwaldzki Square 49, 50-366 Wrocław, Poland
Wojciech Niżański
Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Grunwaldzki Square 49, 50-366 Wrocław, Poland
Joanna Kochan
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture, Mickiewicza 24/28, 30-059 Krakow, Poland
Monika Bugno-Poniewierska
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture, Mickiewicza 24/28, 30-059 Krakow, Poland
Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.
