The dynamic of the early acute aseptic inflammation of testes after transplantation of cryoconservated placenta.

Abstract

In this work the study of the influence of subcutaneous transplantation of cryoconservated placenta on the experimental acute aseptic inflammation of rat testes was carried. The research was carried on 65 Vistar male rats. The experimental aseptic inflammation was caused by intraperitoneal injection of 5 mg -caraginen (“Sigma Co”) in 1 ml 0,9% solution of sodium chloride. The transplantation of cryoconservated placenta by a single subcutaneous injection was made. Testes were taken in 6, 12, 24 hours and on 2nd, 3rd, 5th, 7th, 10th day after beginning of aseptic inflammation and after placenta injection. Thin sections were stained by metilen blue. It was determined that on 7th day after the beginning of experiment the phenomenon of edema of connective tissue diminished. The blood supply at the microcirculatory level was renewed on 7-10th day of experiment. The destruction, desqumation, discomplexation of spermatogonia, spermatocytes I and II, spermatids have dimin-ished also by this term. On a 10th day the gradual renewal of cellular layers and wholeness of basal parts of epithelium of seminiferous tubules was determined. Thus, after injection of placenta there is renewal of cellular composition of spermato-genic epithelium in testes with aseptic inflammation.

Keywords

• -caraginen
• cryoconservated placenta
• aseptic inflammation
• testis
• spermatogenic epithelium
• transplantation of placenta