Generation of Duchenne muscular dystrophy patient-specific induced pluripotent stem cell line lacking exons 45–50 of the dystrophin gene (IITi001-A)

Binyamin Eisen; Ronen Ben Jehuda; Ashley J. Cuttitta; Lucy N. Mekies; Irina Reiter; Sindhu Ramchandren; Michael Arad; Daniel E. Michele; Ofer Binah

Stem Cell Research (May 2018)

Generation of Duchenne muscular dystrophy patient-specific induced pluripotent stem cell line lacking exons 45–50 of the dystrophin gene (IITi001-A)

Binyamin Eisen,
Ronen Ben Jehuda,
Ashley J. Cuttitta,
Lucy N. Mekies,
Irina Reiter,
Sindhu Ramchandren,
Michael Arad,
Daniel E. Michele,
Ofer Binah

Affiliations

Binyamin Eisen: Department of Physiology, Biophysics and Systems Biology, Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, Israel
Ronen Ben Jehuda: Department of Physiology, Biophysics and Systems Biology, Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, Israel; Department of Biotechnology, Technion – Israel Institute of Technology, Haifa, Israel
Ashley J. Cuttitta: Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA
Lucy N. Mekies: Department of Physiology, Biophysics and Systems Biology, Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, Israel
Irina Reiter: Department of Physiology, Biophysics and Systems Biology, Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, Israel
Sindhu Ramchandren: Department of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA
Michael Arad: Leviev Heart Center, Sheba Medical Center, Ramat Gan, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
Daniel E. Michele: Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA; Correspondence to: D. Michele, Department of Molecular and Integrative Physiology, Department of Internal Medicine, University of Michigan North Campus Research, Complex 2800 Plymouth Road, Bldg 26, Room 207S, Ann Arbor, MI 48109-2800, USA.
Ofer Binah: Department of Physiology, Biophysics and Systems Biology, Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, Israel; Correspondence to: O. Binah, Cardiac Research Laboratory, Department of Physiology, Biophysics and Systems Biology, Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, 1 Efron Street, POB 9649, Haifa 31096, Israel.

Journal volume & issue: Vol. 29
pp. 111 – 114

Abstract

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Duchenne muscular dystrophy (DMD) is an X-linked progressive muscle degenerative disease caused by mutations in the dystrophin gene. We generated induced pluripotent stem cells (iPSCs) from a 13-year-old male patient carrying a deletion mutation of exons 45–50; iPSCs were subsequently differentiated into cardiomyocytes. iPSCs exhibit expression of the pluripotent markers (SOX2, NANOG, OCT4), differentiation capacity into the three germ layers, normal karyotype, genetic identity to the skin biopsy dermal fibroblasts and the patient-specific dystrophin mutation.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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