CRISPR-like sequences association with antibiotic resistance and biofilm formation in Helicobacter pylori clinical isolates
Leila Yousefi,
Hiva Kadkhoda,
Masoud Shirmohammadi,
Seyyed Yaghoub Moaddab,
Reza Ghotaslou,
Tahereh pirzadeh,
Javid Sadeghi,
Mohammad Hossein Somi,
Mohammad Ahangarzadeh Rezaee,
Khudaverdi Ganbarov,
Hossein Samadi Kafil
Affiliations
Leila Yousefi
Student Research Committee, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Hiva Kadkhoda
Drug Applied Research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Masoud Shirmohammadi
Liver and Gastrointestinal Diseases Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Seyyed Yaghoub Moaddab
Liver and Gastrointestinal Diseases Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Reza Ghotaslou
Drug Applied Research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Tahereh pirzadeh
Stem Cell Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Javid Sadeghi
Drug Applied Research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad Hossein Somi
Liver and Gastrointestinal Diseases Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad Ahangarzadeh Rezaee
Stem Cell Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Khudaverdi Ganbarov
Research Laboratory of Microbiology and Virology, Baku State University, Z. Khalilov str., 23 AZ1148, Baku, Azerbaijan
Hossein Samadi Kafil
Drug Applied Research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Corresponding author. Tabriz University of Medical Sciences, Tabriz, Iran.
Role of clustered regularly interspaced short palindromic repeats (CRISPR)-like sequences in antibiotic resistance and biofilm formation isn't clear. This study investigated association of CRISPR-like sequences with antibiotic resistance and biofilm formation in H. pylori isolates. Thirty-six of H. pylori isolates were studied for existence of CRISPR-like sequences using PCR method and their correlation with biofilm formation and antibiotic resistance. Microtiter-plate technique was utilized for investigating antibiotic resistance profile of isolates against amoxicillin, tetracycline, metronidazole and clarithromycin. Biofilm formation of isolates was analyzed by microtiter-plate-based-method. Out of 23 CRISPR-like positive isolates, 19 had ability of biofilm formation and 7 of 13 CRISPR-like negative isolates were able to form biofilm (Pvalue = 0.445). In CRISPR-like positive isolates, 11 (48%), 18 (78%), 18 (78%) and 23 (100%) were resistant to amoxicillin, tetracycline, metronidazole and clarithromycin, respectively. Since CRISPR-like sequences have role in antibiotic resistance, may be applied as genetic markers of antibiotic resistance. But there was no substantial correlation between biofilm formation and existence of CRISPR-like sequences. These results indicate possible importance of CRISPR-like sequences on acquisition of resistance to antibiotics in this bacterium.
