The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis

Abstract

ObjectiveThe function of Th17 cells in the neuroinflammatory process in multiple sclerosis has been clarified. The production of IL-17 is dependent on the gene expression of RORc. It has been suggested that Quercetin can influence MS due to a variety of anti-inflammatory effects. Acetylation can enhance lipophilia that is an important feature to cross the Blood-Brain-Barrier, so Quercetin Penta Acetate may cause more improvement for patients. The aim of the present study was to examine in vitro immunomodulatory aspect of Quercetin Penta Acetate as a modified compound on Th17 cells of MS patients, also compared with Quercetin and Methyl Prednisolone Acetate.Materials and MethodsIn present experimental study, PBMCs isolated and stained CFSE then, IC50 values were determined using different doses and times for Quercetin Penta Acetate, and Methyl Prednisolone Acetate. Th17 cells proliferation by flow cytometry and gene expression of IL-17 and RORc by real-time PCR method were analyzed.ResultsThe results showed that IL-17A gene expression was inhibited by Quercetin (P = 0.0081), , and Quercetin Penta Acetate did not have a significant inhibitory effect on Th17 cells proliferation (P= 0.59) and RORc gene expression (P =0.1). Our results showed some immunomodulatory aspects of Quercetin Penta Acetate on Th17 cells is more effective than Quercetin (P= 0.018).ConclusionTaken together, it suggests that Quercetin Penta Acetate can be considered in control MS because of its more potent immunomodulatory properties on Th17 cells compare with Quercetin in some aspects and or as a natural based might be listed among other medications in MS.

Keywords

• multiple sclerosis
• prednisolone
• quercetin
• th17 cells