Population Genetic Quality Analysis of Microsatellite DNA in Wistar Rats Based on T/CALAS 21—2017

Abstract

ObjectiveTo analyze the genetic quality of an outbred stock of Wistar rats in different periods by T/CALAS 21—2017 method, and to evaluate the applicability of the association standard.MethodsWistar rats selected from the same outbred stock in 2015 and 2019 were named as group A and group B, respectively. Twenty-five pairs of microsatellite primers were used to get genetic parameters for quality analysis of the outbred stock Wistar rats according to the association standard T/CALAS 21—2017. Polymorphism information content (PIC) was used to analyze the polymorphism of the loci.ResultsOne hundred alleles in group A and 69 alleles in group B were obtained. The average heterozygosity of the two groups was 0.574 and 0.447, while the average PIC was 0.541 and 0.393, respectively.ConclusionThe association standard T/CALAS 21—2017 has good applicability in the genetic quality analysis of outbred stock rats, and the genetic diversity of group A is better than that of group B.

Keywords

• wistar rats
• t/calas 21—2017
• outbred stock
• microsatellite dna
• genetic diversity