Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans
Alejandro Mata-Cabana,
Olga Sin,
Renée Seinstra,
Ellen Nollen
Affiliations
Alejandro Mata-Cabana
Department of Genetics, University of Seville, Seville, Spain
Olga Sin
Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Münster, GermanyCells-in-Motion Cluster of Excellence, University of Münster, Münster, Germany
Renée Seinstra
European Research Institute for the Biology of Ageing, University of Groningen, University Medical Centre Groningen, Groningen, the Netherlands
Ellen Nollen
European Research Institute for the Biology of Ageing, University of Groningen, University Medical Centre Groningen, Groningen, the Netherlands
C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins.
