Peroxide-induced oxidative stress in erythrocytes under the action of low-frequency vibration

Abstract

Background. In recent years, many publications have highlighted the role of erythrocytes in the pathogenesis of various acute and chronic diseases. Their negative impact is explained by the ability of these cells to generate superoxide anion-radical and other reactive oxygen species (ROS) due to autooxidation of hemoglobin, which increases in hypoxia. The purpose of this work was to study the role of autooxidation of hemoglobin of erythrocytes and activation of redox processes in the regulation of specific physiological processes of these cells under the influence of vibration – a factor that combines mechanical influence, oxidative stress and hypoxia. Materials and Methods. An erythrocyte suspension at T = 25 °C was subjected to vibration for 3 hours in the frequency range from 8 to 32 Hz, with amplitudes of 0.5±0.04 and 0.9±0.08 mm. At specified intervals of time, the content of hydrogen peroxide, the propensity of hemoglobin to autoxidation and the content of hemoglobin ligand forms of the cytoplasmic fraction in the hemolysates of cells were measured. Spearman’s non-parametric correlation analysis was used to analyze the relationship between the studied indicators. Results. The processes of hemoglobin autooxidation in erythrocytes under low-frequen­cy vibration conditions were investigated. Changes in the kinetics of the reaction of erythrocyte hemoglobin oxidation with potassium hexacyanoferrate were observed. An increase in the content of intracellular hydrogen peroxide was shown, which increased more than twice in the frequency range of 16–24 Hz, A = 0.9±0.08 mm. The formation of hemichromes, an increase in the content of methemoglobin in cells was shown. In the frequency range of 20–32 Hz, the formation of ferrylhemoglobin was recorded. Conclusion. Under the influence of vibration in the frequency range of 8–24 Hz, unstable forms of hemoglobin are formed in erythrocytes, which are oxidized to hemichromes. The process of hemoglobin autooxidation, which initiates oxidative stress, slows down over time due to the increase in the content of oxyhemoglobin. The formation of hemichromes at high frequencies indicates the involvement of hemoglobin in oxidative processes, which can have negative consequences for cells.

Keywords

• ligand forms of hemoglobin
• autoxidation of hemoglobin
• heme
• hypoxia
• hemichrome
• ferrylyhemoglobin
• membrane-bound hemoglobin