Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks
Shiyu Wu,
Xinrui Liu,
Yaxin Liu,
Shikai Wang,
Wei Peng,
Ming Zhang,
Binglin Yue,
Hui Wang,
Jikun Wang,
Jincheng Zhong,
Fang Sun,
Yixi Kangzhu,
Jiabo Wang
Affiliations
Shiyu Wu
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Xinrui Liu
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Yaxin Liu
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Shikai Wang
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Wei Peng
Qinghai Academy of Animal Science and Veterinary Science, Qinghai University, Xining 810016, China
Ming Zhang
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Binglin Yue
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Hui Wang
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Jikun Wang
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Jincheng Zhong
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Fang Sun
Key Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture, Institute of Animal-Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150028, China
Yixi Kangzhu
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Jiabo Wang
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, China
Methionine is an amino acid necessary for the growth and development of all animals. Glutathione produced during methionine metabolism can reduce damage to cells caused by oxidative stress. Supplementing restricted amino acids in animals by scientific means will be beneficial to protein synthesis, which will affect the growth and development of animals and will bring huge economic benefits when applied to actual production and life. In this study, we collected three muscle tissues from 24 male Maiwa yaks, which were fattened for three months with different methionine concentrations in their diet. RNA-seq was performed to obtain expression reads. A total of 1116 editing sites were identified by at least two software; the editing site types were mainly T-to-C and A-to-G mutations. We found two significant RNA editing sites presenting high-risk editing types. One was located on the MSRA gene that regulates the reduction of methionine, and the other can make changes to the properties of encoded proteins. This provides further understanding of the mechanism of yak muscle tissue and regulation of gene expression after the addition of methionine to daily rations.
