Chemotactic behavior of egg mitochondria in response to sperm fusion in mice

Maki Iwai; Yuichirou Harada; Rinako Miyabayashi; Woojin Kang; Akihiro Nakamura; Natsuko Kawano; Yoshitaka Miyamoto; Mitsutoshi Yamada; Toshio Hamatani; Mami Miyado; Keiichi Yoshida; Hidekazu Saito; Mamoru Tanaka; Akihiro Umezawa; Kenji Miyado

Heliyon (Nov 2018)

Chemotactic behavior of egg mitochondria in response to sperm fusion in mice

Maki Iwai,
Yuichirou Harada,
Rinako Miyabayashi,
Woojin Kang,
Akihiro Nakamura,
Natsuko Kawano,
Yoshitaka Miyamoto,
Mitsutoshi Yamada,
Toshio Hamatani,
Mami Miyado,
Keiichi Yoshida,
Hidekazu Saito,
Mamoru Tanaka,
Akihiro Umezawa,
Kenji Miyado

Affiliations

Maki Iwai: Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan; Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan
Yuichirou Harada: Department of Molecular Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 192-0397, Japan; Corresponding author.
Rinako Miyabayashi: Shinbashi YUME Clinic, 2-5-1 Shinbashi, Miyato, Tokyo 105-0004, Japan
Woojin Kang: Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan; Department of Perinatal Medicine and Oocyte Care, National Center for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan
Akihiro Nakamura: Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan; Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan
Natsuko Kawano: Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan; Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama, Kawasaki, Kanagawa 214-8571, Japan
Yoshitaka Miyamoto: Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan
Mitsutoshi Yamada: Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan
Toshio Hamatani: Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan
Mami Miyado: Department of Molecular Endocrinology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan
Keiichi Yoshida: Advanced Medicine, Innovation and Clinical Research Center, Tottori University Hospital, 36-1 Nishicho, Yonago, Tottori 683-8504, Japan
Hidekazu Saito: Department of Perinatal Medicine and Oocyte Care, National Center for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan
Mamoru Tanaka: Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan
Akihiro Umezawa: Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan
Kenji Miyado: Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan; Corresponding author.

Journal volume & issue: Vol. 4, no. 11
p. e00944

Abstract

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Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca2+ storage and were colocalized with endoplasmic reticulum (ER) during Ca2+ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca2+ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca2+ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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