Incorporation of Superparamagnetic Iron Oxide Nanoparticles into Collagen Formulation for 3D Electrospun Scaffolds
Manuel Estévez,
Giorgia Montalbano,
Alvaro Gallo-Cordova,
Jesús G. Ovejero,
Isabel Izquierdo-Barba,
Blanca González,
Clarissa Tomasina,
Lorenzo Moroni,
María Vallet-Regí,
Chiara Vitale-Brovarone,
Sonia Fiorilli
Affiliations
Manuel Estévez
Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
Giorgia Montalbano
Department of Applied Science and Technology, Politecnico di Torino, 10129 Torino, Italy
Alvaro Gallo-Cordova
Department of Energy Environment and Health, Instituto de Ciencia de Materiales de Madrid C.S.I.C., Sor Juana Inés de la Cruz 3, Cantoblanco, 28049 Madrid, Spain
Jesús G. Ovejero
Department of Energy Environment and Health, Instituto de Ciencia de Materiales de Madrid C.S.I.C., Sor Juana Inés de la Cruz 3, Cantoblanco, 28049 Madrid, Spain
Isabel Izquierdo-Barba
Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
Blanca González
Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
Clarissa Tomasina
Complex Tissue Regeneration Department, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ET Maastricht, The Netherlands
Lorenzo Moroni
Complex Tissue Regeneration Department, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ET Maastricht, The Netherlands
María Vallet-Regí
Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
Chiara Vitale-Brovarone
Department of Applied Science and Technology, Politecnico di Torino, 10129 Torino, Italy
Sonia Fiorilli
Department of Applied Science and Technology, Politecnico di Torino, 10129 Torino, Italy
Nowadays, there is an ever-increasing interest in the development of systems able to guide and influence cell activities for bone regeneration. In this context, we have explored for the first time the combination of type-I collagen and superparamagnetic iron oxide nanoparticles (SPIONs) to design magnetic and biocompatible electrospun scaffolds. For this purpose, SPIONs with a size of 12 nm were obtained by thermal decomposition and transferred to an aqueous medium via ligand exchange with dimercaptosuccinic acid (DMSA). The SPIONs were subsequently incorporated into type-I collagen solutions to prove the processability of the resulting hybrid formulation by means of electrospinning. The optimized method led to the fabrication of nanostructured scaffolds composed of randomly oriented collagen fibers ranging between 100 and 200 nm, where SPIONs resulted distributed and embedded into the collagen fibers. The SPIONs-containing electrospun structures proved to preserve the magnetic properties of the nanoparticles alone, making these matrices excellent candidates to explore the magnetic stimuli for biomedical applications. Furthermore, the biological assessment of these collagen scaffolds confirmed high viability, adhesion, and proliferation of both pre-osteoblastic MC3T3-E1 cells and human bone marrow-derived mesenchymal stem cells (hBM-MSCs).
