Short Communiation: Effect of different salinity concentration on kidney of benni, Barbus sharpeyi

Abstract

The aim of this study was to investigate the effects of different salinity on kidney of Barbus sharpeyi. For this study, 144 healthy Barbus sharpeyi with an average weight of 350 ± 2.36 grams and length 25 ± 1.25 cm in five groups were studied. The first group as control located in municipal dechlorination water and the next four groups respectively were kept in salinity 4ppt, 8ppt, 12ppt and 16ppt in the same condition. On days 1 , 3 , 7 , 14 , 21 and 28 sample of kidney with maximum thickness of 0.5 cm prepare and were placed in bouin's solution.Then the standard method of parafin sections were done and 5- 6 micrometer thick of tissue sections prepared and stained with H&E methods. Results showed the gradual transfer of fish to water with high salinity caused obvious changes as increase the number and diameter of the glomeruli especially in high salinity but the severity was reduced at the end of the period (p<0.05). Also highest diameter and thickness of the collecting tubules were reported in fresh water at 28 days (p<0.05). Due to the decrease of freshwater resources, from this research can be concluded that the Barbus sharpeyi how much can resist opposite the salinity.These findings suggest that fish Barbus sharpeyi was friendly with salinity and ability to set vital to different salinity

Keywords

• Fish; Histology; Salinity; Gradation