Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing

Abstract

Jiao Fan, Yige Ding, et al. examine the impact of cytosine and adenine deaminases on transcriptome-wide gene expression and splicing in HeLa and HEK293T cells. They found that both kinds of editors could induce broad changes in expression and splicing, independent of Cas9 activity, highlighting the need for further efforts to characterize deaminase enzymes in base editor platforms.