Therapeutic Advances in Chronic Disease (Apr 2020)

Targeted metabolomic analysis of serum phospholipid and acylcarnitine in the adult Fontan patient with a dominant left ventricle

  • Miriam Michel,
  • Karl-Otto Dubowy,
  • Manuela Zlamy,
  • Daniela Karall,
  • Mark Gordian Adam,
  • Andreas Entenmann,
  • Markus Andreas Keller,
  • Jakob Koch,
  • Irena Odri Komazec,
  • Ralf Geiger,
  • Christina Salvador,
  • Christian Niederwanger,
  • Udo Müller,
  • Sabine Scholl-Bürgi,
  • Kai Thorsten Laser

DOI
https://doi.org/10.1177/2040622320916031
Journal volume & issue
Vol. 11

Abstract

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Background: Patients with a Fontan circulation have altered cholesterol and lipoprotein values. We analysed small organic molecules in extended phopsholipid and acylcarnitine metabolic pathways (‘metabolomes’) in adult Fontan patients with a dominant left ventricle, seeking differences between profiles in baseline and Fontan circulations. Methods: In an observational matched cross-sectional study, we compared phosphatidylcholine (PC), sphingomyelin (SM), and acylcarnitine metabolomes (105 analytes; AbsoluteIDQ ® p180 kit (Biocrates Life Sciences AG, Innsbruck, Austria) in 20 adult Fontan patients having a dominant left ventricle with those in 20 age- and sex-matched healthy controls. Results: Serum levels of total PC ( q -value 0.01), total SM ( q -value 0.0002) were significantly lower, and total acylcarnitines ( q -value 0.02) were significantly higher in patients than in controls. After normalisation of data, serum levels of 12 PC and 1 SM Fontan patients were significantly lower ( q -values <0.05), and concentrations of 3 acylcarnitines were significantly higher than those in controls ( q -values <0.05). Conclusion: Metabolomic profiling can use small specimens to identify biomarker patterns that track derangement in multiple metabolic pathways. The striking alterations in the phospholipid and acylcarnitine metabolome that we found in Fontan patients may reflect altered cell signalling and metabolism as found in heart failure in biventricular patients, chronic low-level inflammation, and alteration of functional or structural properties of lymphatic or blood vessels. Trial registration number: ClinicalTrials.gov Identifier NCT03886935