Frontiers in Microbiology (Oct 2022)
Advances in the differential molecular diagnosis of vesicular disease pathogens in swine
- Wenxian Chen,
- Wenxian Chen,
- Weijun Wang,
- Weijun Wang,
- Xinyan Wang,
- Xinyan Wang,
- Zhaoyao Li,
- Zhaoyao Li,
- Keke Wu,
- Keke Wu,
- Xiaowen Li,
- Xiaowen Li,
- Yuwan Li,
- Yuwan Li,
- Yuwan Li,
- Lin Yi,
- Lin Yi,
- Lin Yi,
- Mingqiu Zhao,
- Mingqiu Zhao,
- Mingqiu Zhao,
- Hongxing Ding,
- Hongxing Ding,
- Hongxing Ding,
- Shuangqi Fan,
- Shuangqi Fan,
- Jinding Chen,
- Jinding Chen
Affiliations
- Wenxian Chen
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Wenxian Chen
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Weijun Wang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Weijun Wang
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Xinyan Wang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Xinyan Wang
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Zhaoyao Li
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Zhaoyao Li
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Keke Wu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Keke Wu
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Xiaowen Li
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Xiaowen Li
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Yuwan Li
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yuwan Li
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Yuwan Li
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Lin Yi
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Lin Yi
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Lin Yi
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Mingqiu Zhao
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Mingqiu Zhao
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Mingqiu Zhao
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Hongxing Ding
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Hongxing Ding
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Hongxing Ding
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China
- Shuangqi Fan
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Shuangqi Fan
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Jinding Chen
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Jinding Chen
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2022.1019876
- Journal volume & issue
-
Vol. 13
Abstract
Foot-and-mouth disease virus (FMDV), Senecavirus A (SVA) and swine vesicular disease virus (SVDV) are members of the family Picornaviridae, which can cause similar symptoms - vesicular lesions in the tissues of the mouth, nose, feet, skin and mucous membrane of animals. Rapid and accurate diagnosis of these viruses allows for control measures to prevent the spread of these diseases. Reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR are traditional and reliable methods for pathogen detection, while their amplification reaction requires a thermocycler. Isothermal amplification methods including loop-mediated isothermal amplification and recombinase polymerase amplification developed in recent years are simple, rapid and do not require specialized equipment, allowing for point of care diagnostics. Luminex technology allows for simultaneous detection of multiple pathogens. CRISPR-Cas diagnostic systems also emerging nucleic acid detection technologies which are very sensitivity and specificity. In this paper, various nucleic acid detection methods aimed at vesicular disease pathogens in swine (including FMDV, SVA and SVDV) are summarized.
Keywords
