Effects of Interaperitoneal Administration of α-Tocopherol Acetate on Ischemia-Reperfusion Injury in Ovaries in Female Cats of Animal House Shelter Undergoing Elective Ovariohysterectomy

Abstract

Effects of interaperitoneal administration of α-tocopherol acetate on ischemia-reperfusion injury in ovaries torsion and detorsion were studied using a cat ovary model. Twenty healthy female DSH cats ~3 kg were randomized into five experimental groups (n = 5): Group SHAM: The cats underwent only celiotomy. Group Ischemia: A 3-hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. Group I/ATA: A 3-hour ischemia only and 10 mg/kg intraperitoneal administration (IP) of α-tocopherol 2.5 hours after induction of ischemia. Group I/R/ATA: A 3-hour ischemia, 3-hour reperfusion, and 10 mg/kg IP of α-tocopherol 2.5 hours after induction of ischemia. Animals treated with ATA showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (p < 0.05). The significantly higher values of SOD, tGSH, GPO, GSHRd, and GST were observed in I/R/ATA animals compared to those of other groups (p = 0.001). Damage indicators (NOS, MDA, MPO, and DNA damage level) were significantly lower in I/R/ATA animals compared to those of other groups (p = 0.001). Intraperitoneal administration of ATA could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.

Keywords

• ischemia-reperfusion
• α -tocopherol acetate
• intraperitoneal
• ovary